                         SEQUENCE LISTING

<110>  LINCOLN VENTURES LIMITED
 
<120>  NOVEL BIOSENSOR AND METHOD OF USE

<130>  660924HCF

<150>  US 61/527,754
<151>  2011-08-26

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  84
<212>  DNA
<213>  Escherichia coli

<400>  1
cattaattcc taatttttgt tgacactcta tcattgatag agttatttta ccactcccta       60

tcagtgatag agaaaagtga aatg                                              84


<210>  2
<211>  400
<212>  DNA
<213>  Escherichia coli

<400>  2
cgatagccac tgccgccagt tgacctggta cattcgccag aaagggaatg taatcggcat       60

tttgtccgcc gttaagtgag tgaaattggg tgtaagcctg atccactgcc tgctgtaatt      120

tgtttgcatc taacatcttt tgttaactcc tttttataga tgcgggaggt aattcctcac      180

cccggtgccg attttcaggc atcctgattt aacttagcac ccgcaactta actacaggaa      240

aacaaagaga taaatgtcta atcctgatgc aaatcgagcc gattttttaa tctttacgga      300

cttttacccg cctggtttat taatttcttg accttcccct tgctggaagg tttaaccttt      360

atcacagcca gtcaaaactg tcttaaagga gtgttttatg                            400


<210>  3
<211>  147
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Ser Met Ser Thr Ser Thr Glu Val Ile Ala His His Trp Ala Phe 
1               5                   10                  15      


Ala Ile Phe Leu Ile Val Ala Ile Gly Leu Cys Cys Leu Met Leu Val 
            20                  25                  30          


Gly Gly Trp Phe Leu Gly Gly Arg Ala Arg Ala Arg Ser Lys Asn Val 
        35                  40                  45              


Pro Phe Glu Ser Gly Ile Asp Ser Val Gly Ser Ala Arg Leu Arg Leu 
    50                  55                  60                  


Ser Ala Lys Phe Tyr Leu Val Ala Met Phe Phe Val Ile Phe Asp Val 
65                  70                  75                  80  


Glu Ala Leu Tyr Leu Phe Ala Trp Ser Thr Ser Ile Arg Glu Ser Gly 
                85                  90                  95      


Trp Val Gly Phe Val Glu Ala Ala Ile Phe Ile Phe Val Leu Leu Ala 
            100                 105                 110         


Gly Leu Val Tyr Leu Val Arg Ile Gly Ala Leu Asp Trp Thr Pro Ala 
        115                 120                 125             


Arg Ser Arg Arg Glu Arg Met Asn Pro Glu Thr Asn Ser Ile Ala Asn 
    130                 135                 140                 


Arg Gln Arg 
145         


<210>  4
<211>  444
<212>  DNA
<213>  Escherichia coli

<400>  4
atgagtatgt caacatccac tgaagtcatc gctcatcact gggcattcgc tatctttctt       60

atcgttgcca ttggcctgtg ttgcctgatg ctggtaggcg gttggttttt aggcggtcgc      120

gcacgcgcga ggtcgaaaaa cgtgccgttt gaatccggta tcgactcggt cggctccgcc      180

cgcttacgcc tgtccgccaa gttttatctg gtggccatgt tcttcgttat cttcgacgtt      240

gaagcgctgt atctgttcgc atggtcaacc tctatccgcg aaagcggctg ggtaggcttt      300

gtggaagctg caatttttat ttttgtgtta ctggcaggtc tggtttatct ggtgcgtatt      360

ggcgcgctgg actggacgcc cgcgcgttca cgccgcgagc gtatgaaccc ggaaacgaac      420

agtatcgcta atcgtcaacg ctaa                                             444


<210>  5
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  5
ataggtcgac cagcttttgt tccctttagt g                                      31


<210>  6
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  6
atgagtcgac caattcgccc tatagtgagt                                        30


<210>  7
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  7
atagctgcag actgcccgct ttccagtcgg                                        30


<210>  8
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  8
aggaaaagct tatgaccatg attacgccaa gc                                     32


<210>  9
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  9
atcacctgca gaatgggaat tgacgttcct tc                                     32


<210>  10
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  10
aatcaaagct tttttctcta tcactgatag gga                                    33


<210>  11
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  11
aatcaaagct tttttctcta tcactgatag gga                                    33


<210>  12
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  12
ataggtcgac cagcttttgt tccctttagt g                                      31


<210>  13
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  13
atcaaagctt atgacaaccg aaacgcgttc                                        30


<210>  14
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  14
ataggtcgac cagagatatc gcgcaatacc                                        30


<210>  15
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  15
aatcaaagct tttttctcta tcactgatag gga                                    33


<210>  16
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  16
ataggtcgac cagcttttgt tccctttagt g                                      31


<210>  17
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  17
atcaaagctt atgagtatgt caacatccac t                                      31


<210>  18
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  18
ataggtcgac tcaaccggga tgaatttatc g                                      31


<210>  19
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  19
atagctgcag actgcccgct ttccagtcgg                                        30


<210>  20
<211>  33
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  20
aatcaaagct tttttctcta tcactgatag gga                                    33


<210>  21
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  21
atcacctgca gattttgtcc gccgttaagt g                                      31


<210>  22
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  22
tgacaagctt cactccttta agacagtttt gact                                   34


<210>  23
<211>  362
<212>  PRT
<213>  Escherichia coli

<400>  23

Met Met Lys Arg Asn Ile Leu Ala Val Ile Val Pro Ala Leu Leu Val 
1               5                   10                  15      


Ala Gly Thr Ala Asn Ala Ala Glu Ile Tyr Asn Lys Asp Gly Asn Lys 
            20                  25                  30          


Val Asp Leu Tyr Gly Lys Ala Val Gly Leu His Tyr Phe Ser Lys Gly 
        35                  40                  45              


Asn Gly Glu Asn Ser Tyr Gly Gly Asn Gly Asp Met Thr Tyr Ala Arg 
    50                  55                  60                  


Leu Gly Phe Lys Gly Glu Thr Gln Ile Asn Ser Asp Leu Thr Gly Tyr 
65                  70                  75                  80  


Gly Gln Trp Glu Tyr Asn Phe Gln Gly Asn Asn Ser Glu Gly Ala Asp 
                85                  90                  95      


Ala Gln Thr Gly Asn Lys Thr Arg Leu Ala Phe Ala Gly Leu Lys Tyr 
            100                 105                 110         


Ala Asp Val Gly Ser Phe Asp Tyr Gly Arg Asn Tyr Gly Val Val Tyr 
        115                 120                 125             


Asp Ala Leu Gly Tyr Thr Asp Met Leu Pro Glu Phe Gly Gly Asp Thr 
    130                 135                 140                 


Ala Tyr Ser Asp Asp Phe Phe Val Gly Arg Val Gly Gly Val Ala Thr 
145                 150                 155                 160 


Tyr Arg Asn Ser Asn Phe Phe Gly Leu Val Asp Gly Leu Asn Phe Ala 
                165                 170                 175     


Val Gln Tyr Leu Gly Lys Asn Glu Arg Asp Thr Ala Arg Arg Ser Asn 
            180                 185                 190         


Gly Asp Gly Val Gly Gly Ser Ile Ser Tyr Glu Tyr Glu Gly Phe Gly 
        195                 200                 205             


Ile Val Gly Ala Tyr Gly Ala Ala Asp Arg Thr Asn Leu Gln Glu Ala 
    210                 215                 220                 


Gln Pro Leu Gly Asn Gly Lys Lys Ala Glu Gln Trp Ala Thr Gly Leu 
225                 230                 235                 240 


Lys Tyr Asp Ala Asn Asn Ile Tyr Leu Ala Ala Asn Tyr Gly Glu Thr 
                245                 250                 255     


Arg Asn Ala Thr Pro Ile Thr Asn Lys Phe Thr Asn Thr Ser Gly Phe 
            260                 265                 270         


Ala Asn Lys Thr Gln Asp Val Leu Leu Val Ala Gln Tyr Gln Phe Asp 
        275                 280                 285             


Phe Gly Leu Arg Pro Ser Ile Ala Tyr Thr Lys Ser Lys Ala Lys Asp 
    290                 295                 300                 


Val Glu Gly Ile Gly Asp Val Asp Leu Val Asn Tyr Phe Glu Val Gly 
305                 310                 315                 320 


Ala Thr Tyr Tyr Phe Asn Lys Asn Met Ser Thr Tyr Val Asp Tyr Ile 
                325                 330                 335     


Ile Asn Gln Ile Asp Ser Asp Asn Lys Leu Gly Val Gly Ser Asp Asp 
            340                 345                 350         


Thr Val Ala Val Gly Ile Val Tyr Gln Phe 
        355                 360         


<210>  24
<211>  1089
<212>  DNA
<213>  Escherichia coli

<400>  24
atgatgaagc gcaatattct ggcagtgatc gtccctgctc tgttagtagc aggtactgca       60

aacgctgcag aaatctataa caaagatggc aacaaagtag atctgtacgg taaagctgtt      120

ggtctgcatt atttttccaa gggtaacggt gaaaacagtt acggtggcaa tggcgacatg      180

acctatgccc gtcttggttt taaaggggaa actcaaatca attccgatct gaccggttat      240

ggtcagtggg aatataactt ccagggtaac aactctgaag gcgctgacgc tcaaactggt      300

aacaaaacgc gtctggcatt cgcgggtctt aaatacgctg acgttggttc tttcgattac      360

ggccgtaact acggtgtggt ttatgatgca ctgggttaca ccgatatgct gccagaattt      420

ggtggtgata ctgcatacag cgatgacttc ttcgttggtc gtgttggcgg cgttgctacc      480

tatcgtaact ccaacttctt tggtctggtt gatggcctga acttcgctgt tcagtacctg      540

ggtaaaaacg agcgtgacac tgcacgccgt tctaacggcg acggtgttgg cggttctatc      600

agctacgaat acgaaggctt tggtatcgtt ggtgcttatg gtgcagctga ccgtaccaac      660

ctgcaagaag ctcaacctct tggcaacggt aaaaaagctg aacagtgggc tactggtctg      720

aagtacgacg cgaacaacat ctacctggca gcgaactacg gtgaaacccg taacgctacg      780

ccgatcacta ataaatttac aaacaccagc ggcttcgcca acaaaacgca agacgttctg      840

ttagttgcgc aataccagtt cgatttcggt ctgcgtccgt ccatcgctta caccaaatct      900

aaagcgaaag acgtagaagg tatcggtgat gttgatctgg tgaactactt tgaagtgggc      960

gcaacctact acttcaacaa aaacatgtcc acctatgttg actacatcat caaccagatc     1020

gattctgaca acaaactggg cgtaggttca gacgacaccg ttgctgtggg tatcgtttac     1080

cagttctaa                                                             1089


<210>  25
<211>  972
<212>  DNA
<213>  Escherichia coli

<400>  25
gccattgccc ttgtttatca atgcccagca gcacaacgtc gaagcgactt ttatcaatgg       60

catcgacaat gttttttgcc gattgcagag acacttcatg ttccgctgat ttaccaccaa      120

aaacgattcc tacccgcagt ttttccatct taaaaaccta tcccgtctaa cacaaagtgc      180

atacattacc acgacaaaac gggggattcg cggccttctg aaagattgtt gcaatcttct      240

gctgacaaag cgtgcaacgt actggtgaag aaagtgcgtt atctcaaaga tgtgcgcaag      300

atcacaaaaa tgatgaacgg gaagctaatt tattcctggc ttaaatggcc atgcggtgag      360

tttttttctc ttaattataa gttaacgaag agaatatatt tcataacttt tatttataat      420

aaaggttgat aattaaaagc ctatattttg tgtgggtaat tatttaaata agagaaacgt      480

ttcgctggta atcaaacaaa aaatatttgc gcaaagtatt tcctttgtca taaaaataat      540

acttccagac actatgaagt tgtgaaacat aatgttaact tctccatact ttggataagg      600

aaatacagac atgaaaaatc tcattgctga gttgttattt aagcttgccc aaaaagaaga      660

agagtcgaaa gaactgtgtg cgcaggtaga agctttggag attatcgtca ctgcaatgct      720

tcgcaatatg gcgcaaaatg accaacagcg gttgattgat caggtagagg gggcgctgta      780

cgaggtaaag cccgatgcca gcattcctga cgacgatacg gagctgctgc gcgattacgt      840

aaagaagtta ttgaagcatc ctcgtcagta aaaagttaat cttttcaaca gctgtcataa      900

agttgtcacg gccgagactt atagtcgctt tgtttttatt ttttaatgta tttgtacatg      960

gagaaaataa ag                                                          972


<210>  26
<211>  859
<212>  DNA
<213>  Escherichia coli

<400>  26
tgccgccggg aagtggtaag ccagaaatgt atgcttctga cctggtgacg ctgttaaaag       60

aagtggcaga tttcgatatc tccgtggcgg cgtatccgga agttcacccg gaagcaaaaa      120

gcgctcaggc ggatttgctt aatctgaaac gcaaagtgga tgccggagcc aaccgcgcga      180

ttactcagtt cttcttcgat gtcgaaagct acctgcgttt tcgtgaccgc tgtgtatcgg      240

cgggcattga tgtggaaatt attccgggaa ttttgccggt atctaacttt aaacaggcga      300

agaaatttgc cgatatgacc aacgtgcgta ttccggcgtg gatggcgcaa atgttcgacg      360

gtctggatga tgatgccgaa acccgcaaac tggttggcgc gaatattgcc atggatatgg      420

tgaagatttt aagccgtgaa ggagtgaaag atttccactt ctatacgctt aaccgtgctg      480

aaatgagtta cgcgatttgc catacgctgg gggttcgacc tggtttataa atagtgtggc      540

ttttgtgaaa atcacacagt gatcacaaat tttaaacaga gcacaaaatg ctgcctcgaa      600

atgagggcgg gaaaataagg ttatcagcct tgttttctcc ctcattactt gaaggatatg      660

aagctaaaac ccttttttat aaagcatttg tccgaattcg gacataatca aaaaagctta      720

attaagatca atttgatcta catctcttta accaacaata tgtaagatct caactatcgc      780

atccgtggat taattcaatt ataacttctc tctaacgctg tgtatcgtaa cggtaacact      840

gtagagggga gcacattga                                                   859


<210>  27
<211>  732
<212>  DNA
<213>  Escherichia coli

<400>  27
catttgcgca aaattattga tgtactcttt taacgacgcg ccctcagggg aaacatccac       60

atcgtggaag caataaaatg gcacatgtaa cttgtggaaa aactcaaatg cgacatctgc      120

tttacgcttc gccaacgcca gtgcctcacc aggctgctgc cacggacgat taaacgcccc      180

cacaccaaac atatccgccc cgttccagca gaaggtgtgc cagtagcagg cggcaaaacg      240

caagtgctct tccatacgct tacccaacac cagttcgtcg ggattgtagt gacggaatgc      300

taacgggttt gaggattttg agccttcata acgaacgcga tcgagctggt caaaataggc      360

ttgcatattg aactccataa tcaggtaatg ccgcgggtga tggatgatgt cgtaatattg      420

ggcactccct ttcagttgct caattatgtt atttcacact gctattgaga taattcacaa      480

gtgtgcgctc gctcgcaaaa taaaatggaa tgatgaaact gggtaattcc tcgaagagaa      540

aaatgcaata agtacaattg cgcaacaaaa gtaagatctc ggtcataaat caagaaataa      600

accaaaaatc gtaatcgaaa gataaaaatc tgtaattgtt ttcccctgtt tagttgctaa      660

aaattggtta cgtttatcgc ggtgattgtt acttattaaa actgtcctct aactacagaa      720

ggccctacac ca                                                          732


<210>  28
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  28
atcagtcgac aatgggaatt gacgttcctt c                                      31


<210>  29
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Primer

<400>  29
atagggatcc tcaaccggga tgaatttatc g                                      31


